T4 DNA Ligase

Specification:20,000 U, 100,000 U
Concentration:20,000 U---400,000 U/ml, 100,000 U---400,000 U/ml
Product description:T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
Product application:
1.Cloning of restriction enzyme generated DNA fragments
2.Joining of double-stranded oligonucleotide linkers or adaptors to DNA