Pullulanase Activity and Applications

Pullulanase is an endo-type enzyme that cleaves α-1,6-glucosidic linkages in polysaccharides, such as amylopectin, glycogen, dextrin and pullulan. Pullulanase requires the aberration alternation to be of at atomic a maltose breadth in adjustment for it to be able to carve the α-1,6 linkage. In addition, abasement of amylopectin by β-amylase occurs in an exo appearance starting from the non-reducing end of the sugar, but the acknowledgment stops afore the α-1,6 linkages. However, if pullulanase is supplied with β-amylase, amylopectin can be base about completely.
Pullulanase is aswell referred to as “debranching enzyme,” because it hydrolyzes the aberration α-1,6-glucosidic linkages in polysaccharides, such as amylopectin. This debranching action of pullulanase is accumulated with the activities of added enzymes, such as β-amylase and isoamylase, for the assembly of maltose and oligosaccharides. Also, assembly of angled cyclodextrins can be performed application the abstract acknowledgment that pullulanase can catalyze.