Exonuclease I (E.coli)
Specification:3,000 U, 15,000 U
Concentration:20,000 U/ml
Product description:Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
Product application:
1.Primer removal from PCR mixtures:
prior to PCR product sequencing
for one-tube "megaprimer" PCR mutagenesis
2.Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
3.Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus
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