Dithiothreitol

CAS: 3483-12-3
Molecular Formula: C4H10O2S2
Molecular Weight: 154.253
Product description:One of the purposes is as thiol DNA of reducing agent and protective agent. Thiol the DNA at the end of sulfur atoms in solution tends to form dimers, especially in the presence of oxygen. This second polymerization greatly reduces the some coupling reaction experiment, such as DNA biosensor in the fixed efficiency; Add DTT in DNA solution, reaction after a period of time to remove, can reduce the polymerization of DNA. DTT is also often used in the reduction of disulfide bonds in proteins that can be used to prevent protein formed between the cysteine protein intramolecular or intermolecular disulfide bonds. But DTT is often unable to restore the embedding within the protein structure (solvent) and disulfide bond, the reduction of disulfide bond is often necessary to protein denaturation first (high temperature heating or join denaturant, such as 6 m guanidine hydrochloride, 8 m urea or 1% SDS).