Acridine Orange

CAS: 10127-02-3
Molecular Formula: C34H40Cl4N6Zn
Molecular Weight: 739.9426
Product description:Orange powder. Soluble in water, ethanol. Solution for green fluorescent orange belt. PH value of 8.4 ~ 10.4, no fluorescence. Can set in between two adjacent base pairs, the release part of the DNA double helix, the two bases of distance widened. Such DNA replication process, can increase the DNA strand or missing a base, causing frameshift mutations.Used as a fluorescence tracer, tumor cells and bacteria, nucleic acid stain and mutagen frameshift mutations. Acridine orange is a kind of fluorescent pigment, acridine orange filter wavelength of 488 nm. Blocking filter wavelength of 515 nm. Acridine orange combined with DNA and RNA in the cell volume there is a difference, but a different color fluorescent specificity (shaded), this is because the DNA is a high polymer, absorb the location of the fluorescent material is less, hair green fluorescence, the low degree of polymerization, and RNA can and fluorescent material combined with the location of the many, so the hair red fluorescence.