RNase H

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RNase H

Specification:250 U, 1250 U
Concentration:5,000 U/ml
Product description:Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids (see Figure 1 in Supporting Data). It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Product application:
1.Removal of mRNA prior to synthesis of second strand cDNA
2.RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
3.Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
4.Site-specific cleavage of RNA
5.Studies of in vitro polyadenylation reaction products


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